LipoSEARCH - Conditions and Instructions for Order and Sample Shipping

Sample volume required and dilution rate

Serum: dilution is not necessary if 45µL or more is available.

Leave the sample at room temperature for 30 min after serum collection. Subsequently, after cooling the sample on ice, centrifuge at 3000 rpm for 15 min at 4°C. Solidified chylomicrons are often observed in the top separating layer in samples obtained from the fat tolerance test. In such a case, dissolve these thoroughly using a pipette. Use of a blood collection tube with clot activator will facilitate pipetting because the layer of clot activator inserts between the serum and blood clot layer.

Plasma: dilution is not necessary if 45µL or more is available.

After collection in a plasma separation tube with clotting factor inhibitor, the sample and the inhibitor are mixed by inversion so that they can react sufficiently. After cooling the sample on ice, the plasma is separated by centrifugation at 3000 rpm for 15 min at 4 °C. For samples obtained from the fat tolerance test, please follow the same procedure as that used with serum samples but use a plasma separation tube containing separating agents.

*For samples of low concentration, such as culture supernatants and cerebrospinal fluid, please contact us before the dispatch.

N.B.

1. If only a small quantity is available, please send a 1:20 dilution of 500µL (25µL of the sample diluted with saline to 500µL).
2. If the volume of diluted sample and the dilution ratio is different for some reason, please state this clearly on the order sheet explained in the next slide.
3. samples containing anticoagulant (* Heparin is NOT acceptable as an anticoagulant) or samples that may contain pathogens.
- Frozen samples are measurable, but the very TG-rich ones are not acceptable. Freezing and thawing should only occur once.

Effects of freezing and thawing on triglyceride level

Effects of freezing and thawing on Cholesterol level

Sample Shipping

-Supply the sample in a 0.5 to 2.0µL microtube for centrifugation (e.g., Eppendorf tube) and firmly seal it with Parafilm.
-Write the ID on each sample tube (e.g., sample name and/or number) using an indelible pen.
-Ask the shipping company to keep your samples refrigerated.
-Pack cold insulators or dry ice along with your samples.
-For fresh samples, avoid direct contact with the insulators or dry ice in order to prevent freezing during the transportation.

N.B.

1. We are NOT responsible for the sample preparation, the arrangement of shipping, any mishandling by the shipping company during the transportation (e.g., spillage during the transportation), and any resultant loss.
2. We will NOT store and/or return your samples.
3. Samples that are repeatedly frozen and thawed are not accepted.

Consignee/Shipping address

Analysis Center, Skylight Biotech Inc.
100-4 Sunada, Iijima
Akita-shi, Akita 011-0911 JAPAN
TEL:+81-18-880-5060

※The shipping schedule should be arranged so that the samples can be delivered to the analysis center on weekdays or Saturday. We cannot receive them on Sunday or any national holiday in Japan.

Order sheet

-Once you have arranged the shipping, please fax the order sheet to +81-3-5207-6578.
-Please provide the following information in the order sheet while placing an order:
Type, origin (species, e.g., human, mice/sex/age), dilution rate, and any other background of the samples (e.g., if they are taken from patients, please state the name of disease).
-Whether you need data on 4 fractions and/or 20 fractions.