How to Order & Sample Volume Requirement

How to Order

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How to Make Your Order

Download the Order Sheet (Excel format) first.
Download Order Sheet

Once you have arranged sample shipping, fill out the Order Sheet and attach to your e-Mail
Mail to: globalinfo@skylight-biotech.com

Provide the following information in your Order Sheet: Type, Origin (species, e.g. human, mice/sex/age), and any other background of your samples (e.g. if they are taken from patients, state the name of disease).

Our agent will contact you to support your ordering procedure.

Workflow – From Order to Payment

Sample Volume Requirement

Samples should be either Serum or Plasma. Volume required is no less than 45µL for human samples, and no less than 35µL for animal samples. Please contact us in advance if your sample types are other than serum or plasma.

Serum: human 45µL or more; animal 35µL or more (Undiluted)
Leave the sample at room temperature for 30 min. after blood collection. Subsequently, after cooling the sample on ice, centrifuge at 3,000 rpm for 15 min. at 4ºC. Solidified chylomicrons are observed in the top separating layer in samples obtained from the fat tolerance test. In such case, dissolve these thoroughly using a pipette. Use of a blood collection tube with clot activator will facilitate pipetting because the layer of clot activator inserts between serum and blood clot layer.

Serum: human 45µL or more; animal 35µL or more (Undiluted)
After collection in a plasma separation tube with clotting factor inhibitor, the sample and the inhibitor are mixed by inversion so that they can react sufficiently. After cooling the sample on ice, the plasma is separated by centrifugation at 3,000 rpm for 15 min at 4ºC. For samples obtained from the fat tolerance test, please follow the same procedure as that used with serum samples but use a plasma separation tube containing separating agents.

For samples of low concentration, such as culture supernatants and cerebrospinal fluid, please contact us before shipping.

N.B.

In case that 45µL (human) or 35µL (animal) is not available, contact us in advance.

Contact us in advance if you require analysis of samples containing anticoagulant (* Heparin is NOT acceptable as an anticoagulant) or samples that may contain pathogens.

We recommend that samples be frozen immediately after the collection and stored at -80 ºC until the shipment. Samples that are repeatedly frozen and thawed are not accepted.

Pooled samples should be avoided.

Filter sterilization is recommended in order to avoid denaturation of lipoprotein by bacteria-derived protease.

Negative Effects of Freezing and Thawing

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