Less Sample Enables Time-course Strudies

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Time-course Studies While Keeping Test Animals Alive

Lipoprotein profile testing with LipoSEARCH needs just a drop of serum/plasma. Conventional testing methods like ultracentrifugation and electrophoresis ask for a certain volume of sample blood. In testing with small lab animals like high triglyceride mouse, a client has to pool sample blood collected from multiple targets to secure the needed quantity.

With only 10μL serum/plasma injection,  LipoSEARCH delivers complete and accurate data on cholesterol and triglyceride. This makes it possible for researchers to perform time-course studies on lipoprotein profiles while keeping valuable experimental animals alive.

Wild-type mouse   Apo E knock-out mouse
HDL of a wild-type mouse does not contain TG. Since CETP activity is very low in wild-type mice, a small amount of HDL-C is transferred to VLDL and LDL while a small amount of TG from VLDL and LDL is transferred to HDL. A broad VLDL peak is observed. It appears that several small VLDLs remain in blood as VLDL remnants and are not taken into the liver due to Apo E deficiency.
   
LCAT knock-out mouse Human CETP-expressing mouse
Free cholesterols are not esterified to produce cholesterol esters because lecithin cholesterol acyltransferase (LCAT) is deficient. Therefore, HDL cannot mature, and the total cholesterol level is lowered. A human CETP transgenic mouse showed a HDL-TG peak because TG from VLDL and LDL is transferred to HDL. On the other hand, the HDL-C level decreased as HDL-C is transferred to VLDL and LDL.
   
Wild-type rabbit (JW) Ref. Cholesterol, TG, free cholesterol and Phospholipid monitors (Wild type mouse)
The sizes of LDL and HDL are similar to those in humans. The TG levels of LDL and HDL are higher than those in humans because of the high CETP activity. Free cholesterol (FC) and PL are also measurable in addition to cholesterol and TG ().
100µL or more of sample is required.

Wild Mouse vs. Diabetes Model – Analysis Samples

LipoSEARCH chromatogram helps researchers to visually check the differences and changes in lipid metabolism.

(Wild) C57BL/6,8 week-old ♂   (Diabetes Model) KK-Ay,9 week-old ♂
   
Wister 9-week ♂ Zucker 10-week ♂

Dogs – Normal, Obesity and Diabetes – Analysis Samples

Normal   Obesity   Diabetes
Mostly HDL on samples from healthy dogs and cats. LDL increases as they become obese. Cholesterol and TG of CM and VLDL increase when they suffer from diabetes, cushing syndrome and thyroid dysfunction.

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