Since only 10µL of the sample is required for the analysis, time-course studies are possible while keeping your important test animals alive.
When using existing methods such as ultracentrifugation and gel electrophoresis, test animals have to be sacrificed and some samples need to be pooled in order to obtain sufficient amounts for the analyses.
Apo E knock-out mouse
Pattern No. 45 from SLB library
A broad VLDL peak is observed. It appears that several small VLDLs remain in blood as VLDL remnants and are not taken into the liver due to Apo E deficiency.
Wild-type mouse
Pattern No. 42 from SLB library
HDL of a wild-type mouse does not contain TG. Since CETP activity is very low in wild-type mice, a small amount of HDL-C is transferred to VLDL and LDL while a small amount of TG from VLDL and LDL is transferred to HDL.
Human CETP-expressing mouse
Pattern No. 44 from SLB library
A human CETP transgenic mouse showed a HDL-TG peak because TG from VLDL and LDL is transferred to HDL. On the other hand, the HDL-C level decreased as HDL-C is transferred to VLDL and LDL.
LCAT knock-out mouse
Pattern No. 43 from SLB library
Free cholesterols are not esterified to produce cholesterol esters because lecithin cholesterol acyltransferase (LCAT) is deficient. Therefore, HDL cannot mature, and the total cholesterol level is lowered.
Ref. Cholesterol, TG, free cholesterol, and PL monitors (example: wild-type mouse)
Free cholesterol (FC) and PL are also measurable in addition to cholesterol and TG (*).
* 100µL or more of sample is required.
Wild-type rabbit (JW)
Pattern No. 4 from SLB library
The sizes of LDL and HDL are similar to those in humans. The TG levels of LDL and HDL are higher than those in humans because of the high CETP activity.
>> Examples of analysis of human samples